Re: alocasia DNA sample 1

From: "Julius Boos" ju-bo at msn.com> on 2002.03.04 at 21:25:18(8253) Dear Ben, I am NOT good at Alocasias, but in Deni Bown`s second edition of her book 'Aroids--Plants of the Arum Family', Alocasia 'Tigrina superba' is considered but a 'cultivar' of Alocasia zebrina (pg 184) . Julius +More >>Denis of Silver Krome Gardens Homestead Florida was so kind to send me leaf a samples of 11 Alocasias. I measured their DNA and got t some interesting results I will treat them in small groups for you so as to invite your comment as I dont know anything about Alocasias . General: A sample of leaf was chopped with the standard Agave americana of 15.9 pg Nuclei were""colored"" with propidium iodide and measured one by one in a flow cytometer. About 5000 to 10.000 nuclei were measured and the values in Alocasia range from 10 -22 picogram Furhter I noted that three samples had very dark suspensions. I found 11.2 picogram per nucleus for Alocasia tigrina superba and 11.6 pg for A zebrina reticulata . These values are too close for comfort They might be closely related species or Could they be the same species? Ben J.M.Zonneveld Clusius lab pobox 9505 2300 RA Leiden The Netherlands mintemp-16C(5F) Zonneveld@RULbim.LeidenUniv.NL Fax: 31-71-5274999

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