From: ted.held at us.henkel.com (ted.held at us.henkel.com) on 2008.01.10 at 18:26:37(16942)
Dear Surawit,
Thank you for your reply. It looks like there is much information"out
there" about this science that has been hidden from me all these years. I
think I'll try Leland's advice as well, and think about what you have
written.
My response is humble and I have a number of new questions now that will
only add to my perplexity. Here are a few.
1. How reliable are chromosome counts, statistically? How many cells do
you need to count before you are satisfied that you have the right number?
2. Is it possible to have a nuisance cell or two that show a different
count from others from the same plant? I mean, what do you do if you
count, say 20 cells with 2n = 40 and one with 2n = 42?
3. How easy is it to break a chromosome in the process of performing the
preparation and root tip crush?
I'd better stop now.
Ted.
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