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Tissue Culture ? Can common folk do it?
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From: santoury at aol.com on 2010.09.09 at 21:54:00(21426)
I'm sure this has been answered before, but nothing on the internet shows any "common folk" doing tissue culture work to propagate their own plants. I'd love to do it, if possible. Granted, my facilities are not "scientific." But, boy, if I could grow plants from pieces of leaves - I'd sure love to.
I'd love to hear about any experience you may have had with this endeavor.
Thanks! Jude
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From: "plantguy at zoominternet.net" <plantguy at zoominternet.net> on 2010.09.09 at 23:09:55(21428)
Hi Jude,
I must admit, I have often thought of trying it in my lab which is complete
with anything that would be necessary, but have never found the time or
actual inclination to have a stab at it.
There are a lot of sites on the internet that show you how to do this as a
home-TC person however. Sterility will be the biggest problem, but can be
solved with 70% EtOH and a home-made hood.....also a alcohol burner. I've
always thought the biggest issue would be which formulation to make for the
agar you are doing the TC on as there are a million possible supplements
that can be added (the agar also needs to be sterilized which is typically
done in an autoclave).
Best of luck if you give it a try and I would be interested in hearing your
results.
| +More |
Dan
Gibsonia, PA
Original Message:
-----------------
From: santoury@aol.com
Date: Thu, 09 Sep 2010 17:54:00 -0400
To: aroid-l@www.gizmoworks.com, pbs@lists.ibiblio.org
Subject: [Aroid-l] Tissue Culture ? Can common folk do it?
I'm sure this has been answered before, but nothing on the internet shows
any "common folk" doing tissue culture work to propagate their own plants.
I'd love to do it, if possible. Granted, my facilities are not
"scientific." But, boy, if I could grow plants from pieces of leaves - I'd
sure love to.
I'd love to hear about any experience you may have had with this endeavor.
Thanks! Jude
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From: Don Martinson <llmen at wi.rr.com> on 2010.09.09 at 23:57:10(21430)
In general, tissue culture is a very exacting and not inexpensive endeavor and not really intended for (no offense intended) us “common folk”. I’m not saying it can’t be done, but I don’t believe it is easily achieved without specialized materials and lots of expert help.
Don Martinson
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Milwaukee, Wisconsin
Mailto:llmen@wi.rr.com
On 9/9/10 4:54 PM, "santoury@aol.com" wrote:
I'm sure this has been answered before, but nothing on the internet shows any "common folk" doing tissue culture work to propagate their own plants. I'd love to do it, if possible. Granted, my facilities are not "scientific." But, boy, if I could grow plants from pieces of leaves - I'd sure love to.
I'd love to hear about any experience you may have had with this endeavor.
Thanks! Jude
_______________________________________________
Aroid-L mailing list
Aroid-L@www.gizmoworks.com
http://www.gizmoworks.com/mailman/listinfo/aroid-l
--B_3366903431_1702375--
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From: "Sherry Gates" <TheTropix at msn.com> on 2010.09.10 at 00:33:13(21431)
I excitedly 2nd this request!
thanks, sherry
----- Original Message -----
| HTML +More |
From: santoury@aol.com
To: aroid-l@www.gizmoworks.com ; pbs@lists.ibiblio.org
Sent: Thursday, September 09, 2010 4:54 PM
Subject: [Aroid-l] Tissue Culture ? Can common folk do it?
I'm sure this has been answered before, but nothing on the internet shows any "common folk" doing tissue culture work to propagate their own plants. I'd love to do it, if possible. Granted, my facilities are not "scientific." But, boy, if I could grow plants from pieces of leaves - I'd sure love to.
I'd love to hear about any experience you may have had with this endeavor.
Thanks! Jude
_______________________________________________
Aroid-L mailing list
Aroid-L@www.gizmoworks.com
http://www.gizmoworks.com/mailman/listinfo/aroid-l
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From: "StroWi at t-online.de" <StroWi at t-online.de> on 2010.09.10 at 05:31:01(21433)
Jude,
there is a yahoo group on hometissueculture...
http://tech.groups.yahoo.com/group/hometissueculture/
| +More |
Happy growing, Bernhard.
-----Original Message-----
> Date: Thu, 09 Sep 2010 23:54:00 +0200
> Subject: [Aroid-l] Tissue Culture ? Can common folk do it?
> From: santoury@aol.com
> To: aroid-l@www.gizmoworks.com, pbs@lists.ibiblio.org
> I'm sure this has been answered before, but nothing on the internet
shows any "common folk" doing tissue culture work to propagate their own
plants. I'd love to do it, if possible. Granted, my facilities are not
"scientific." But, boy, if I could grow plants from pieces of leaves -
I'd sure love to. I'd love to hear about any experience you may have had
with this endeavor. Thanks! Jude
_______________________________________________
Aroid-L mailing list
Aroid-L@www.gizmoworks.com
http://www.gizmoworks.com/mailman/listinfo/aroid-l
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From: Zach DuFran <zdufran at wdtinc.com> on 2010.09.10 at 13:55:21(21435)
Jude-
One of our IAS members (Ghazanfar Ghori) has a very nice
blog where he documents his work with Cryptocorynes. He does a lot of
tissue culture. Check it out:
http://kryptokoryne.aquaticscape.com/
Zach
| HTML +More |
From:
aroid-l-bounces@www.gizmoworks.com [mailto:aroid-l-bounces@www.gizmoworks.com] On Behalf Of santoury@aol.com
Sent: Thursday, September 09, 2010
4:54 PM
To: aroid-l@www.gizmoworks.com;
pbs@lists.ibiblio.org
Subject: [Aroid-l] Tissue Culture
? Can common folk do it?
I'm sure this has been answered before,
but nothing on the internet shows any "common folk" doing tissue
culture work to propagate their own plants. I'd love to do it, if possible.
Granted, my facilities are not "scientific." But, boy, if I could
grow plants from pieces of leaves - I'd sure love to.
I'd love to hear about any experience you
may have had with this endeavor.
Thanks! Jude
--_000_792F4A6158EAE44189316A2EAD8CCBB91D675Drainwdtinccom_--
--==============e27905302172083030= |
|
From: Steve Marak <samarak at gizmoworks.com> on 2010.09.11 at 17:29:35(21443)
My wife's setup involves a pressure cooker, an electronic kitchen balance
that reads in grams, and a "hood" made by buying a large clear plastic bin
at Wal-Mart, turning it upside down, and cutting a hole in the side and
taping a clear plastic flap over it. After trying several options, she's
now using regular home canning jars (with the plastic lids, not the metal
ones) to hold the medium.
We happened to already have the pressure cooker and electronic kitchen
balance, but other than those, the most expensive thing has been
purchasing the media, which Cathy gets from PhytoTech (
http://www.phytotechlab.com ). She's buying pre-mixed formulations they
offer, which require only the addition of sugar.
At the moment she's focused on learning to flask orchid seed efficiently,
rather than tissue culture, but she has done a little TC in that kind of
setup when she was teaching high school, so it can be done.
Carolina Biological ( http://www.carolina.com ) offers TC "kits" designed
for teachers/students/home enthusiasts (put "tissue culture" in the search
box). They won't set you up to do TC of your plants at home, but can give
you a feel for the kind of lab techniques you need (and help give you the
confidence that you can do it), and several of them are pretty cheap - the
sundew TC kit is less than $25 US.
Sterilization of the environment in the hood is key, of course. Some
people use a spray bottle of ethanol, but not only is it very "fumey",
it's very flammable, so Cathy uses other options. You can use dilute
bleach, or some people prefer a calcium hypochlorite (household bleach is
a sodium hypochlorite solution) solution instead. Aaron Hicks, at his
Orchid Seedbank Project, has lots of great advice from his experience
flasking orchids which would apply in TC also. Check out
http://members.cox.net/ahicks51/osp/
Steve
| +More |
On Thu, 9 Sep 2010, Sherry Gates wrote:
> I excitedly 2nd this request!
> thanks, sherry
> ----- Original Message -----
> From: santoury@aol.com
> Subject: [Aroid-l] Tissue Culture ? Can common folk do it?
>
> I'm sure this has been answered before, but nothing on the internet
> shows any "common folk" doing tissue culture work to propagate their
> own plants. I'd love to do it, if possible. Granted, my facilities are
> not "scientific." But, boy, if I could grow plants from pieces of
> leaves - I'd sure love to. I'd love to hear about any experience you
> may have had with this endeavor. Thanks! Jude
-- Steve Marak
-- samarak@gizmoworks.com
_______________________________________________
Aroid-L mailing list
Aroid-L@www.gizmoworks.com
http://www.gizmoworks.com/mailman/listinfo/aroid-l
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From: santoury at aol.com on 2010.09.11 at 21:01:20(21445)
Hey there
Thank you very much - Here I was thinking, hoping, that it would just be a matter of cutting up some leaves, and putting them on agar... The whole spraying, chemicals and such - no thanks! Darn. One could only hope, right? :)
| HTML +More |
-----Original Message-----
From: Steve Marak
To: Discussion of aroids
Sent: Sat, Sep 11, 2010 1:29 pm
Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it?
My wife's setup involves a pressure cooker, an electronic kitchen balance that reads in grams, and a "hood" made by buying a large clear plastic bin at Wal-Mart, turning it upside down, and cutting a hole in the side and taping a clear plastic flap over it. After trying several options, she's now using regular home canning jars (with the plastic lids, not the metal ones) to hold the medium.
We happened to already have the pressure cooker and electronic kitchen balance, but other than those, the most expensive thing has been purchasing the media, which Cathy gets from PhytoTech ( http://www.phytotechlab.com ). She's buying pre-mixed formulations they offer, which require only the addition of sugar.
At the moment she's focused on learning to flask orchid seed efficiently, rather than tissue culture, but she has done a little TC in that kind of setup when she was teaching high school, so it can be done.
Carolina Biological ( http://www.carolina.com ) offers TC "kits" designed for teachers/students/home enthusiasts (put "tissue culture" in the search box). They won't set you up to do TC of your plants at home, but can give you a feel for the kind of lab techniques you need (and help give you the confidence that you can do it), and several of them are pretty cheap - the sundew TC kit is less than $25 US.
Sterilization of the environment in the hood is key, of course. Some people use a spray bottle of ethanol, but not only is it very "fumey", it's very flammable, so Cathy uses other options. You can use dilute bleach, or some people prefer a calcium hypochlorite (household bleach is a sodium hypochlorite solution) solution instead. Aaron Hicks, at his Orchid Seedbank Project, has lots of great advice from his experience flasking orchids which would apply in TC also. Check out http://members.cox.net/ahicks51/osp/
Steve
On Thu, 9 Sep 2010, Sherry Gates wrote:
> I excitedly 2nd this request!
> thanks, sherry
> ----- Original Message ----- > From: santoury@aol.com > Subject: [Aroid-l] Tissue Culture ? Can common folk do it?
> > I'm sure this has been answered before, but nothing on the internet > shows any "common folk" doing tissue culture work to propagate their > own plants. I'd love to do it, if possible. Granted, my facilities are > not "scientific." But, boy, if I could grow plants from pieces of > leaves - I'd sure love to. I'd love to hear about any experience you > may have had with this endeavor. Thanks! Jude
-- Steve Marak
-- samarak@gizmoworks.com
_______________________________________________
Aroid-L mailing list
Aroid-L@www.gizmoworks.com
http://www.gizmoworks.com/mailman/listinfo/aroid-l
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--==============29508571198032742= |
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From: "Steve Hatfield" <moondogman at comcast.net> on 2010.09.11 at 21:11:37(21446)
http://www.hometissueculture.org/
I found this a few months ago.It looks doable on a budget. I think a
tinkerer could come up with most of the things in the kits.
Check out these videos on YouTube Micropropagation Part I Intro.
http://www.youtube.com/watch?v=KYvJByYrSPg
looks doable.
Steve
| +More |
-----Original Message-----
From: aroid-l-bounces@www.gizmoworks.com
[mailto:aroid-l-bounces@www.gizmoworks.com] On Behalf Of StroWi@t-online.de
Sent: Friday, September 10, 2010 1:31 AM
To: Discussion of aroids
Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it?
Jude,
there is a yahoo group on hometissueculture...
http://tech.groups.yahoo.com/group/hometissueculture/
Happy growing, Bernhard.
-----Original Message-----
> Date: Thu, 09 Sep 2010 23:54:00 +0200
> Subject: [Aroid-l] Tissue Culture ? Can common folk do it?
> From: santoury@aol.com
> To: aroid-l@www.gizmoworks.com, pbs@lists.ibiblio.org
> I'm sure this has been answered before, but nothing on the internet
shows any "common folk" doing tissue culture work to propagate their own
plants. I'd love to do it, if possible. Granted, my facilities are not
"scientific." But, boy, if I could grow plants from pieces of leaves -
I'd sure love to. I'd love to hear about any experience you may have had
with this endeavor. Thanks! Jude
_______________________________________________
Aroid-L mailing list
Aroid-L@www.gizmoworks.com
http://www.gizmoworks.com/mailman/listinfo/aroid-l
_______________________________________________
Aroid-L mailing list
Aroid-L@www.gizmoworks.com
http://www.gizmoworks.com/mailman/listinfo/aroid-l
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|
From: "plantguy at zoominternet.net" <plantguy at zoominternet.net> on 2010.09.11 at 21:12:56(21447)
Hi Steve,
Sounds like your wife is having some fun :o)
I guess I forgot about a nice place to look up info and get a free book on
the subject. If you go to www.sigma-aldrich.com you can find a huge number
of things by putting plant tissue culture in the search box. The only
problem is that you can get overwhelmed by the sheer volume of
info.....beware if you really want scientific info.....there is a massive
amount of it.
| +More |
I actually got this free manual quite awhile ago when I was contemplating
doing this, but as usual, life got in the way.
Here is a link....scroll down and ask for the catalogue....do not know if
you have to be in academia to get it.......I will request one to find out:
http://www.sigmaaldrich.com/life-science/molecular-biology/plant-biotechnolo
gy/learning-center/tissue-culture-guide.html
Needless to say, unless you just want to have fun, you need to have a plant
rare enough to warrant TC.
Dan
Gibsonia, PA
Original Message:
-----------------
From: Steve Marak samarak@gizmoworks.com
Date: Sat, 11 Sep 2010 12:29:35 -0500 (CDT)
To: aroid-l@www.gizmoworks.com
Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it?
My wife's setup involves a pressure cooker, an electronic kitchen balance
that reads in grams, and a "hood" made by buying a large clear plastic bin
at Wal-Mart, turning it upside down, and cutting a hole in the side and
taping a clear plastic flap over it. After trying several options, she's
now using regular home canning jars (with the plastic lids, not the metal
ones) to hold the medium.
We happened to already have the pressure cooker and electronic kitchen
balance, but other than those, the most expensive thing has been
purchasing the media, which Cathy gets from PhytoTech (
http://www.phytotechlab.com ). She's buying pre-mixed formulations they
offer, which require only the addition of sugar.
At the moment she's focused on learning to flask orchid seed efficiently,
rather than tissue culture, but she has done a little TC in that kind of
setup when she was teaching high school, so it can be done.
Carolina Biological ( http://www.carolina.com ) offers TC "kits" designed
for teachers/students/home enthusiasts (put "tissue culture" in the search
box). They won't set you up to do TC of your plants at home, but can give
you a feel for the kind of lab techniques you need (and help give you the
confidence that you can do it), and several of them are pretty cheap - the
sundew TC kit is less than $25 US.
Sterilization of the environment in the hood is key, of course. Some
people use a spray bottle of ethanol, but not only is it very "fumey",
it's very flammable, so Cathy uses other options. You can use dilute
bleach, or some people prefer a calcium hypochlorite (household bleach is
a sodium hypochlorite solution) solution instead. Aaron Hicks, at his
Orchid Seedbank Project, has lots of great advice from his experience
flasking orchids which would apply in TC also. Check out
http://members.cox.net/ahicks51/osp/
Steve
On Thu, 9 Sep 2010, Sherry Gates wrote:
> I excitedly 2nd this request!
> thanks, sherry
> ----- Original Message -----
> From: santoury@aol.com
> Subject: [Aroid-l] Tissue Culture ? Can common folk do it?
>
> I'm sure this has been answered before, but nothing on the internet
> shows any "common folk" doing tissue culture work to propagate their
> own plants. I'd love to do it, if possible. Granted, my facilities are
> not "scientific." But, boy, if I could grow plants from pieces of
> leaves - I'd sure love to. I'd love to hear about any experience you
> may have had with this endeavor. Thanks! Jude
-- Steve Marak
-- samarak@gizmoworks.com
_______________________________________________
Aroid-L mailing list
Aroid-L@www.gizmoworks.com
http://www.gizmoworks.com/mailman/listinfo/aroid-l
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From: "Nancy Greig" <ngreig at hmns.org> on 2010.09.12 at 14:26:26(21453)
I seem to recall that Steve Lucas mentioned to me that Tony Avent had written an article for the IAS journal on growing Amorphophallus plants from leaf cuttings. Does anyone know the issue number?
Nancy Greig
| +More |
Director, Cockrell Butterfly Center
Houston Museum of Natural Science
One Hermann Circle Drive
Houston, TX 77030-1799
tel 713-639-4742
fax 713-639-4788
www.hmns.org
-----Original Message-----
From: aroid-l-bounces@www.gizmoworks.com on behalf of santoury@aol.com
Sent: Sat 9/11/2010 4:01 PM
To: aroid-l@www.gizmoworks.com
Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it?
Hey there
Thank you very much - Here I was thinking, hoping, that it would just be a matter of cutting up some leaves, and putting them on agar... The whole spraying, chemicals and such - no thanks! Darn. One could only hope, right? :)
-----Original Message-----
From: Steve Marak
To: Discussion of aroids
Sent: Sat, Sep 11, 2010 1:29 pm
Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it?
My wife's setup involves a pressure cooker, an electronic kitchen balance
that reads in grams, and a "hood" made by buying a large clear plastic bin
at Wal-Mart, turning it upside down, and cutting a hole in the side and
taping a clear plastic flap over it. After trying several options, she's
now using regular home canning jars (with the plastic lids, not the metal
ones) to hold the medium.
We happened to already have the pressure cooker and electronic kitchen
balance, but other than those, the most expensive thing has been
purchasing the media, which Cathy gets from PhytoTech (
http://www.phytotechlab.com ). She's buying pre-mixed formulations they
offer, which require only the addition of sugar.
At the moment she's focused on learning to flask orchid seed efficiently,
rather than tissue culture, but she has done a little TC in that kind of
setup when she was teaching high school, so it can be done.
Carolina Biological ( http://www.carolina.com ) offers TC "kits" designed
for teachers/students/home enthusiasts (put "tissue culture" in the search
box). They won't set you up to do TC of your plants at home, but can give
you a feel for the kind of lab techniques you need (and help give you the
confidence that you can do it), and several of them are pretty cheap - the
sundew TC kit is less than $25 US.
Sterilization of the environment in the hood is key, of course. Some
people use a spray bottle of ethanol, but not only is it very "fumey",
it's very flammable, so Cathy uses other options. You can use dilute
bleach, or some people prefer a calcium hypochlorite (household bleach is
a sodium hypochlorite solution) solution instead. Aaron Hicks, at his
Orchid Seedbank Project, has lots of great advice from his experience
flasking orchids which would apply in TC also. Check out
http://members.cox.net/ahicks51/osp/
Steve
On Thu, 9 Sep 2010, Sherry Gates wrote:
> I excitedly 2nd this request!
> thanks, sherry
> ----- Original Message -----
> From: santoury@aol.com
> Subject: [Aroid-l] Tissue Culture ? Can common folk do it?
>
> I'm sure this has been answered before, but nothing on the internet
> shows any "common folk" doing tissue culture work to propagate their
> own plants. I'd love to do it, if possible. Granted, my facilities are
> not "scientific." But, boy, if I could grow plants from pieces of
> leaves - I'd sure love to. I'd love to hear about any experience you
> may have had with this endeavor. Thanks! Jude
-- Steve Marak
-- samarak@gizmoworks.com
_______________________________________________
Aroid-L mailing list
Aroid-L@www.gizmoworks.com
http://www.gizmoworks.com/mailman/listinfo/aroid-l
------_=_NextPart_001_01CB5286.B8BFFDBE
_______________________________________________
Aroid-L mailing list
Aroid-L@www.gizmoworks.com
http://www.gizmoworks.com/mailman/listinfo/aroid-l
------_=_NextPart_001_01CB5286.B8BFFDBE--
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From: ExoticRainforest <Steve at ExoticRainforest.com> on 2010.09.12 at 21:42:38(21454)
Nancy, it is in volume 30.
Steve
| HTML +More |
www.ExoticRainforest.com
On 9/12/2010 09:26, Nancy Greig wrote:
I seem to recall that Steve Lucas mentioned to me that Tony Avent had written an article for the IAS journal on growing Amorphophallus plants from leaf cuttings. Does anyone know the issue number?
Nancy Greig
Director, Cockrell Butterfly Center
Houston Museum of Natural Science
One Hermann Circle Drive
Houston, TX 77030-1799
tel 713-639-4742
fax 713-639-4788
www.hmns.org
-----Original Message-----
From: aroid-l-bounces@www.gizmoworks.com on behalf of santoury@aol.com
Sent: Sat 9/11/2010 4:01 PM
To: aroid-l@www.gizmoworks.com
Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it?
Hey there
Thank you very much - Here I was thinking, hoping, that it would just be a matter of cutting up some leaves, and putting them on agar... The whole spraying, chemicals and such - no thanks! Darn. One could only hope, right? :)
-----Original Message-----
From: Steve Marak
To: Discussion of aroids
Sent: Sat, Sep 11, 2010 1:29 pm
Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it?
My wife's setup involves a pressure cooker, an electronic kitchen balance
that reads in grams, and a "hood" made by buying a large clear plastic bin
at Wal-Mart, turning it upside down, and cutting a hole in the side and
taping a clear plastic flap over it. After trying several options, she's
now using regular home canning jars (with the plastic lids, not the metal
ones) to hold the medium.
We happened to already have the pressure cooker and electronic kitchen
balance, but other than those, the most expensive thing has been
purchasing the media, which Cathy gets from PhytoTech (
http://www.phytotechlab.com ). She's buying pre-mixed formulations they
offer, which require only the addition of sugar.
At the moment she's focused on learning to flask orchid seed efficiently,
rather than tissue culture, but she has done a little TC in that kind of
setup when she was teaching high school, so it can be done.
Carolina Biological ( http://www.carolina.com ) offers TC "kits" designed
for teachers/students/home enthusiasts (put "tissue culture" in the search
box). They won't set you up to do TC of your plants at home, but can give
you a feel for the kind of lab techniques you need (and help give you the
confidence that you can do it), and several of them are pretty cheap - the
sundew TC kit is less than $25 US.
Sterilization of the environment in the hood is key, of course. Some
people use a spray bottle of ethanol, but not only is it very "fumey",
it's very flammable, so Cathy uses other options. You can use dilute
bleach, or some people prefer a calcium hypochlorite (household bleach is
a sodium hypochlorite solution) solution instead. Aaron Hicks, at his
Orchid Seedbank Project, has lots of great advice from his experience
flasking orchids which would apply in TC also. Check out
http://members.cox.net/ahicks51/osp/
Steve
On Thu, 9 Sep 2010, Sherry Gates wrote:
I excitedly 2nd this request!
thanks, sherry
----- Original Message -----
From: santoury@aol.com
Subject: [Aroid-l] Tissue Culture ? Can common folk do it?
I'm sure this has been answered before, but nothing on the internet
shows any "common folk" doing tissue culture work to propagate their
own plants. I'd love to do it, if possible. Granted, my facilities are
not "scientific." But, boy, if I could grow plants from pieces of
leaves - I'd sure love to. I'd love to hear about any experience you
may have had with this endeavor. Thanks! Jude
-- Steve Marak
-- samarak@gizmoworks.com
_______________________________________________
Aroid-L mailing list
Aroid-L@www.gizmoworks.com
http://www.gizmoworks.com/mailman/listinfo/aroid-l
_______________________________________________
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From: "Derek Burch" <derek at horticulturist.com> on 2010.09.13 at 02:16:51(21457)
Nancy,
There is a searchable index on Aroid-l (saved me going to the files).
2007 30 124-138 Tony Avent Propagation of Amorphophallus by
leaf petiole cuttings (Buy)
He takes away a lot of the mystery.
I haven't looked back in the Aroid-l archives, but this topic or one like it
has come up in the past. It is worth getting to know all the things on
aroid-l: Aroideana stuff, aroiders, cultivar registration, and many more
gems, not to mention great galleries of images. It is not limited to members
of IAS, although we all hope that it generates membership.
Derek
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-----Original Message-----
From: aroid-l-bounces@www.gizmoworks.com
[mailto:aroid-l-bounces@www.gizmoworks.com] On Behalf Of Nancy Greig
Sent: Sunday, September 12, 2010 10:26 AM
To: Discussion of aroids
Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it?
I seem to recall that Steve Lucas mentioned to me that Tony Avent had
written an article for the IAS journal on growing Amorphophallus plants from
leaf cuttings. Does anyone know the issue number?
Nancy Greig
Director, Cockrell Butterfly Center
Houston Museum of Natural Science
One Hermann Circle Drive
Houston, TX 77030-1799
tel 713-639-4742
fax 713-639-4788
www.hmns.org
-----Original Message-----
From: aroid-l-bounces@www.gizmoworks.com on behalf of santoury@aol.com
Sent: Sat 9/11/2010 4:01 PM
To: aroid-l@www.gizmoworks.com
Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it?
Hey there
Thank you very much - Here I was thinking, hoping, that it would just be a
matter of cutting up some leaves, and putting them on agar... The whole
spraying, chemicals and such - no thanks! Darn. One could only hope,
right? :)
-----Original Message-----
From: Steve Marak
To: Discussion of aroids
Sent: Sat, Sep 11, 2010 1:29 pm
Subject: Re: [Aroid-l] Tissue Culture ? Can common folk do it?
My wife's setup involves a pressure cooker, an electronic kitchen balance
that reads in grams, and a "hood" made by buying a large clear plastic bin
at Wal-Mart, turning it upside down, and cutting a hole in the side and
taping a clear plastic flap over it. After trying several options, she's
now using regular home canning jars (with the plastic lids, not the metal
ones) to hold the medium.
We happened to already have the pressure cooker and electronic kitchen
balance, but other than those, the most expensive thing has been
purchasing the media, which Cathy gets from PhytoTech (
http://www.phytotechlab.com ). She's buying pre-mixed formulations they
offer, which require only the addition of sugar.
At the moment she's focused on learning to flask orchid seed efficiently,
rather than tissue culture, but she has done a little TC in that kind of
setup when she was teaching high school, so it can be done.
Carolina Biological ( http://www.carolina.com ) offers TC "kits" designed
for teachers/students/home enthusiasts (put "tissue culture" in the search
box). They won't set you up to do TC of your plants at home, but can give
you a feel for the kind of lab techniques you need (and help give you the
confidence that you can do it), and several of them are pretty cheap - the
sundew TC kit is less than $25 US.
Sterilization of the environment in the hood is key, of course. Some
people use a spray bottle of ethanol, but not only is it very "fumey",
it's very flammable, so Cathy uses other options. You can use dilute
bleach, or some people prefer a calcium hypochlorite (household bleach is
a sodium hypochlorite solution) solution instead. Aaron Hicks, at his
Orchid Seedbank Project, has lots of great advice from his experience
flasking orchids which would apply in TC also. Check out
http://members.cox.net/ahicks51/osp/
Steve
On Thu, 9 Sep 2010, Sherry Gates wrote:
> I excitedly 2nd this request!
> thanks, sherry
> ----- Original Message -----
> From: santoury@aol.com
> Subject: [Aroid-l] Tissue Culture ? Can common folk do it?
>
> I'm sure this has been answered before, but nothing on the internet
> shows any "common folk" doing tissue culture work to propagate their
> own plants. I'd love to do it, if possible. Granted, my facilities are
> not "scientific." But, boy, if I could grow plants from pieces of
> leaves - I'd sure love to. I'd love to hear about any experience you
> may have had with this endeavor. Thanks! Jude
-- Steve Marak
-- samarak@gizmoworks.com
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