A. Titanum cloned

From: "WEAVER,BILL (HP-USA,ex1)" bill.weaver at hp.com> on 2003.09.15 at 22:21:30(10581) A year or two ago there was some discussion on the list as to whether Amorphophallus titanum could be successfully cloned from a leaf cutting. So I decided to give it a try. I took two leaflets off of the big leaf and treated one with Rootone F and one with Olivia's Cloning Gel. Each leaf was then put in a six inch pot full of seedling mix (fine vermiculite/sand/perlite) and zipped into a plastic bag and placed under fluorescent lights. The leaflets stayed green for almost a year. After they died and rotted I strained the potting material I found something pretty cool. The Rootone treated leaflet had produced three tiny bulblets, the largest was about 9mm and the smallest about 3mm. The Cloning Gel treated leaflet produced the most interesting growth. The midrib of the leaf had produced two cylindrical growths, the smaller one was about 2.5cm long and about 7mm wide. The other was over 6cm long and 2cm wide. Attached to it were two small bulblets 5mm and 15mm in diameter. I planted all of the bulblets and growths in April 2003 and the first new growths sprouted from the Cloning Gel pot this weekend. To put it mildly I am very happy with the results and decided to share the story so far. Bill Weaver +More bill.weaver@hp.com

From: "zach" r2ot at charter.net> on 2003.09.16 at 10:50:19(10583) I also had success with this When my very small a.titanum grew up a leaf it pushed off the old one before it had withered.i dipped it in roottone +More put it into a light medium in a terrariam.About 2 weeks after,it had enough roots to support itself in the pot.

From: StroWi at t-online.de (StroWi) on 2003.09.17 at 08:22:00(10587) Bill, congratulation for your cloning experiment! I remember very well the discussion on the list on titanum leaf cuttings in summer last year. Interesting that you got some what different responses depending on the rooting or cloning powder or gel. Would you have the ingredients or formulation of those products? This would maybe shed a light on the differneces between the two leaf cuttings you grew. Good further cloning, Bernhard. +More Dollbergen, Germany approx. zone 6/7 "WEAVER,BILL (HP-USA,ex1)" schrieb: > A year or two ago there was some discussion on the list as to > whether Amorphophallus titanum could be successfully cloned from a > leaf cutting......... > > Bill Weaver > bill.weaver@hp.com >

From: Harry Witmore harrywitmore at witmore.net> on 2003.09.17 at 18:46:08(10592) --- Outgoing mail is certified Virus Free. Checked by AVG anti-virus system (http://www.grisoft.com). Version: 6.0.518 / Virus Database: 316 - Release Date: 9/11/2003

From: Douglas Ewing dewing at u.washington.edu> on 2003.09.18 at 15:35:59(10598) I too have been cloning titanum from leaf cuttings. We differ from Bill's procedure slightly: I use a 12" portion of the leaf with the mid-vein. I have not experimented with smaller cuttings. We apply no hormone. ( this is something I have avoided with all leaf cuttings in the past, as I was under the impression that auxin would promote root formation, but that it inhibits formation of shoots. Since we want both to happen with a leaf cutting, we do not apply. Tubers are shoots, perhaps this explains the difference in the gel vs. powder results?) We stick the cutting into a 8cm cube of rockwool, water, and encapsulate the entire affair in a terrarium made from 2 2liter pop-bottles. This creates almost total humidity, and cutting do not wilt. If/when fungus appears on the cutting, we dust with cinnamon. Tuber formation takes several months, then we wean from humidity by utilizing a pop-bottle with the bottom removed and the cap off, this allows a bit of air exchange for a few days , then the cutting is removed from the structure, potted up and placed on the open bench. Typically the orig. leaf tissue dies, followed by new leaf emergence. We also see multiple leaf emergence, which I am assuming will result in multiple tubers. I have not tried other sp. of this genus, but the fact that several species produce tubercles on the leaf naturally,( A. bulbifer) makes me think that all sp. might respond to this treatment. Doug +More _________________/\/\/\______________________ Doug Ewing, Greenhouse Manager (206) 543-0436 Department of Biology University of Washington Box 351800 Seattle, WA 98195-1800

From: "Scott Taylor" staylor at brevardparks.com> on 2003.09.18 at 21:01:32(10599) Just so I am clear on this cloning technique: does one use the entire leaf, or trim the lateral material away from the midvein? And how much of the petiole is retained? +More Thanks dst D. Scott Taylor, Ph.D. Brevard County Environmentally Endangered Lands (EEL) Program Central Region Land Manager 5560 North US Highway 1 Melbourne, FL 32940 tel: 321.255.4466 FAX: 321.255.4499 email: staylor@brevardparks.com -----Original Message----- [mailto:aroid-l-owner@lists.ncsu.edu]On Behalf Of Douglas Ewing Sent: Thursday, September 18, 2003 11:36 AM To: 'aroid-l posting' Subject: Re: [aroid-l] A. Titanum cloned I too have been cloning titanum from leaf cuttings. We differ from Bill's procedure slightly: I use a 12" portion of the leaf with the mid-vein. I have not experimented with smaller cuttings. We apply no hormone. ( this is something I have avoided with all leaf cuttings in the past, as I was under the impression that auxin would promote root formation, but that it inhibits formation of shoots. Since we want both to happen with a leaf cutting, we do not apply. Tubers are shoots, perhaps this explains the difference in the gel vs. powder results?) We stick the cutting into a 8cm cube of rockwool, water, and encapsulate the entire affair in a terrarium made from 2 2liter pop-bottles. This creates almost total humidity, and cutting do not wilt. If/when fungus appears on the cutting, we dust with cinnamon. Tuber formation takes several months, then we wean from humidity by utilizing a pop-bottle with the bottom removed and the cap off, this allows a bit of air exchange for a few days , then the cutting is removed from the structure, potted up and placed on the open bench. Typically the orig. leaf tissue dies, followed by new leaf emergence. We also see multiple leaf emergence, which I am assuming will result in multiple tubers. I have not tried other sp. of this genus, but the fact that several species produce tubercles on the leaf naturally,( A. bulbifer) makes me think that all sp. might respond to this treatment. Doug _________________/\/\/\______________________ Doug Ewing, Greenhouse Manager (206) 543-0436 Department of Biology University of Washington Box 351800 Seattle, WA 98195-1800

From: "WEAVER,BILL (HP-USA,ex1)" bill.weaver at hp.com> on 2003.09.19 at 22:02:31(10608) I used just a leaflet one of the little (4" X 8") oval components that make up the entire leaf. I just took one that came to a nice point with very little leaf tissue on either side of the mid-rib where I cut it off. Bill +More -----Original Message----- Sent: Thursday, September 18, 2003 2:02 PM To: aroid-l@lists.ncsu.edu Subject: Re: [aroid-l] A. Titanum cloned Just so I am clear on this cloning technique: does one use the entire leaf, or trim the lateral material away from the midvein? And how much of the petiole is retained? Thanks dst D. Scott Taylor, Ph.D. Brevard County Environmentally Endangered Lands (EEL) Program Central Region Land Manager 5560 North US Highway 1 Melbourne, FL 32940 tel: 321.255.4466 FAX: 321.255.4499 email: staylor@brevardparks.com -----Original Message----- [mailto:aroid-l-owner@lists.ncsu.edu]On Behalf Of Douglas Ewing Sent: Thursday, September 18, 2003 11:36 AM To: 'aroid-l posting' Subject: Re: [aroid-l] A. Titanum cloned I too have been cloning titanum from leaf cuttings. We differ from Bill's procedure slightly: I use a 12" portion of the leaf with the mid-vein. I have not experimented with smaller cuttings. We apply no hormone. ( this is something I have avoided with all leaf cuttings in the past, as I was under the impression that auxin would promote root formation, but that it inhibits formation of shoots. Since we want both to happen with a leaf cutting, we do not apply. Tubers are shoots, perhaps this explains the difference in the gel vs. powder results?) We stick the cutting into a 8cm cube of rockwool, water, and encapsulate the entire affair in a terrarium made from 2 2liter pop-bottles. This creates almost total humidity, and cutting do not wilt. If/when fungus appears on the cutting, we dust with cinnamon. Tuber formation takes several months, then we wean from humidity by utilizing a pop-bottle with the bottom removed and the cap off, this allows a bit of air exchange for a few days , then the cutting is removed from the structure, potted up and placed on the open bench. Typically the orig. leaf tissue dies, followed by new leaf emergence. We also see multiple leaf emergence, which I am assuming will result in multiple tubers. I have not tried other sp. of this genus, but the fact that several species produce tubercles on the leaf naturally,( A. bulbifer) makes me think that all sp. might respond to this treatment. Doug _________________/\/\/\______________________ Doug Ewing, Greenhouse Manager (206) 543-0436 Department of Biology University of Washington Box 351800 Seattle, WA 98195-1800

From: Douglas Ewing dewing at u.washington.edu> on 2003.09.23 at 16:07:38(10620) Bill, it did take several months before new leaves appeared. Doug _________________/\/\/\______________________ Doug Ewing, Greenhouse Manager (206) 543-0436 +More Department of Biology University of Washington Box 351800 Seattle, WA 98195-1800 On Thu, 18 Sep 2003, WEAVER,BILL (HP-USA,ex1) wrote: > What was the timeframe before the new shoots appeared? I had to dig them up > and repot them before anything happened. > > Bill > > -----Original Message----- > From: Douglas Ewing [mailto:dewing@u.washington.edu] > Sent: Thursday, September 18, 2003 8:36 AM > To: 'aroid-l posting' > Subject: Re: [aroid-l] A. Titanum cloned > > > I too have been cloning titanum from leaf cuttings. We differ from Bill's > procedure slightly: > > I use a 12" portion of the leaf with the mid-vein. I have not > experimented with smaller cuttings. > > We apply no hormone. ( this is something I have avoided with all > leaf cuttings in the past, as I was under the impression that auxin would > promote root formation, but that it inhibits formation of shoots. Since we > want both to happen with a leaf cutting, we do not apply. Tubers are > shoots, perhaps this explains the difference in the gel vs. powder > results?) > > We stick the cutting into a 8cm cube of rockwool, water, and > encapsulate the entire affair in a terrarium made from 2 2liter > pop-bottles. This creates almost total humidity, and cutting do not wilt. > If/when fungus appears on the cutting, we dust with cinnamon. Tuber > formation > takes several months, then we wean from humidity by utilizing a pop-bottle > with the bottom removed and the cap off, this allows a bit of air exchange > for a few days , then the cutting is removed from the structure, potted up > and placed on the open bench. Typically the orig. leaf tissue dies, > followed by new leaf emergence. We also see multiple leaf emergence, which > I am assuming will result in multiple tubers. > > I have not tried other sp. of this genus, but the fact that > several species produce tubercles on the leaf naturally,( A. bulbifer) > makes me think that all sp. might respond to this treatment. > > > Doug > _________________/\/\/\______________________ > > Doug Ewing, Greenhouse Manager (206) 543-0436 > Department of Biology > University of Washington > Box 351800 > Seattle, WA 98195-1800 > > > > >

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