Refrigeration of aroid pollen.

From: "Julius Boos" ju-bo at msn.com> on 2003.06.12 at 09:17:49(10338) DEar Friends, I was wondering what information may be out there on the storage of aroid pollen by refrigeration and/or freezing, and the length of time it may remain viable??? Does anyone on these lists have information or literature on this?? There are a couple rare species 'out there' that I am keeping tabs on, and if it were possible to refrigerate or to freeze pollen and then use it at a later date to pollinate a bloom on another plant of the same species even in another state or country, this would be a great thing! This was brought to mind by the recent spate of bloomings of the Amorphophallus titanums, most of which probably were grown from seed collected by the late Dr. James Symon. Good Growing, Julius Boos +More W.P.B., Florida.

From: Marc Gibernau gibernau at cict.fr> on 2003.06.13 at 07:47:17(10339) Dear Julius, I've prepared with Anita Diaz a manuscript for our favourite journal on this subject on two Arum species. But It will take some times before we'll receive the next Aroideana issue. In Arum, to store pollen in refrigerators (8 or 15?C) prolong pollen viability up to 4-5 days. May be at cooler temperatures (3-5?C) the pollen viability will last longer. I think that water freezing will be a (non-return) limit. +More About freezing pollen, I do not know any references but I think that it will kill the pollen (frost may destroy the cellular organs). But it must be tested!!!! On the aroid web site, you can read Scott Hyndman's web page on Amorphophallus pollen stored in a refrogerator. All the best, Marc

From: "Denis Rotolante" denisskg at bellsouth.net> on 2003.06.13 at 13:23:07(10342) You might check with Dr. Jake Henny at the Apopka research station....he's certainly done enough Aroid hybridizing in his time... Denis +More -----Original Message----- On Behalf Of Julius Boos Sent: Thursday, June 12, 2003 5:18 AM To: aroid-l@lists.ncsu.edu Cc: The_bulbous_aroids@yahoogroups.com Subject: Re: [aroid-l] Refrigeration of aroid pollen. DEar Friends, I was wondering what information may be out there on the storage of aroid pollen by refrigeration and/or freezing, and the length of time it may remain viable??? Does anyone on these lists have information or literature on this?? There are a couple rare species 'out there' that I am keeping tabs on, and if it were possible to refrigerate or to freeze pollen and then use it at a later date to pollinate a bloom on another plant of the same species even in another state or country, this would be a great thing! This was brought to mind by the recent spate of bloomings of the Amorphophallus titanums, most of which probably were grown from seed collected by the late Dr. James Symon. Good Growing, Julius Boos W.P.B., Florida.

From: "Julius Boos" ju-bo at msn.com> on 2003.06.16 at 00:14:00(10343) Dear Marc, Thanks to you, Denis and the others who replied. I am looking for some specific methods, temps., etc. with which I may be able to store aroid pollen for a little more than a year. If anyone has more information, please pass it along! Good growing, Julius +More >>>Dear Julius, I've prepared with Anita Diaz a manuscript for our favourite journal on this subject on two Arum species. But It will take some times before we'll receive the next Aroideana issue. In Arum, to store pollen in refrigerators (8 or 15?C) prolong pollen viability up to 4-5 days. May be at cooler temperatures (3-5?C) the pollen viability will last longer. I think that water freezing will be a (non-return) limit. About freezing pollen, I do not know any references but I think that it will kill the pollen (frost may destroy the cellular organs). But it must be tested!!!! On the aroid web site, you can read Scott Hyndman's web page on Amorphophallus pollen stored in a refrogerator. All the best, Marc

Note: this is a very old post, so no reply function is available.