[Aroid-l] =?iso-8859-15?q?=27Indian_Giant=27_ploidylevel_=5Bwas?=

From: "StroWi at t-online.de" <StroWi at t-online.de> on 2011.01.21 at 14:06:30 -----Original Message----- > Date: Wed, 07 Jul 2010 21:03:55 +0200 > Subject: Re: [Aroid-l] Sauromatum x Pinellia hybrid.... and others... > From: James Waddick <jwaddick@kc.rr.com> > To: Discussion of aroids aroid-l@www.gizmoworks.com .... > In this same discussion, the cv "Indian Giant' is brought to > mind. This is a much larger form of S. venosum. Could it be a > tetraploid? Has anyone compared chromosome numbers to the typical, > smaller form? And is this, by the way, any more or less hardier than > the typical form? Dear Jim W., Aroiders, already more than one year ago I compared the ploidy level of a normal form of Typhonium venosum (a genotype/tuber that came from Laos) with my=A0'Indian Giant' clone that was originally introduced by Tony Avent (Plant Delights Nursery). Furthermore I have the clone that was given to me as T. nubicum. but is probably identical with 'Indian Giant'. For pictures see: (scroll down to the post from 06.11.2009) http://www.amorphophallus-forum.de/viewtopic.php?f=3D9&t=3D316&start=3D30 I did not count the chromosomes but used flowcytometry to compare the DNA content. In short words with=A0this method the DNA content of nuclei is measured after the DNA was=A0stained. The measured values are shown in a graph. The first peak represents the cells in the G1-phase; this would give the DNA-content of somatic cells. The second peak represents the nucelar DNA content of cell in the G2-phase after the replication of the DNA before the cell devides. The graphs show the measurements of T. venosum 'Laos', T.venosum 'Indian Giant' and T. nubicum, respectively. [img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_v_Laos.jpg[/img] [img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_v_IndianGiant.jpg[/img] [img]http://i82.photobucket.com/albums/j260/Mr_Titanum/T_nubicum.jpg[/img] The results strongly indicate that the three clones do not differ in DNA content and therefore have the same ploidy level. However, I did no chomosome counts, but if 'Indian Giant' would be a tetraploid of the normal form of T. venosum, the graph should show=A0a first peak at channel 100 for the G1-nuclei and a second at channel 200 for the G2-nulei. This is not the case; all three clones have the peaks=A0roughly at channel 50 and channel 100. Best, Bernhard. --===============4191384668144448325==

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