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Year Vol.
(Issue) Pages Author(s) Title

1997 20 29-36 Adelheid R. Kuehnle Progress in protoplast Isolation and culture from axenic tissues of hybrid Anthurium (Buy)
ABSTRACT: Protoplasts capable of first divisions were isolated from Anthurium andraeanum Linden ex Andre hybrids using axenic etiolated shoots of the University of Hawaii Antburium cultivars 'Rudolph', UH1060, and UH1003. Enzymatic digestion by 1.5% (w/v) Cellulase R-lO, 0.5% Macerozyme R-10 and 0.5% Driselase in 0.5 M mannitol gave consistent yields between 1.6 X 104 to 2.2 X 104 protoplasts per gram fresh weight of etiolated shoots. In contrast, similar digestions using green leaves of micropropagated plantlets always resulted in mixtures of protoplasts and partially digested cells in ratios of about 1:1 to 1:3, respectively, with a mean yield of 3.8 X 105 protoplasts per gram fresh weight of leaves. Etiolated shoots exposed to a 14-day low light treatment gave consistently higher yields than those with no light exposure, with 3.8 X 104 and 1.8 X 104 protoplasts per gram fresh weight, respectively. Anthurium 'Rudolph' and UH1060 protoplasts were cultured in darkness at densities ranging from 2.5 X 104 to 7.0 X 104 protoplasts ml- ' in a basic tobacco protoplast culture medium with 1 mg' liter-l 6-benzylaminopurine, 1 mgÂ·liter-' IX-naphthaleneacetic acid, and 0.1 mgÂ·liter-' 2,4-dichlorophenoxyacetic acid either in liquid or embedded in 0.6% agarose. Antburium etiolated shoot protoplasts divided within 2 to 6 days and some sustained divisions to form microcolonies within 10 days under these conditions.

1999 22 48-61 Nuttha Kuanprasert, Adelheid R. Kuehnle Fragrance quality (Buy)
ABSTRACT: A total of 146 Anthurium species and hybrids from sections Belolonchium, Calomystrium, Cardiolonchium, Chamaerepium, Dactylophyllum, Leptanthurium, Oxycarpium, Pachyneurium, Porphyrochitonium, Semaeophyllium, Tetraspermium and Urospadix was evaluated for floral fragrance. Type of fragrance, time of emission, daily occurrence and developmental stage of scent emission were recorded along with the color of spathe and spadix and the environmental conditions. A majority of plants emitted scent: 68% of the species and 80% of the hybrids were fragrant. Fragrance was categorized as citrus, fishy, floral, foul, fruity, menthol, minty, pine, spicy, and sweet. There was no correlation between scent production or quality with flower color or botanical section. A plurality of plants emitted scent during the morning only (45%) and at the pistillate stage (77%). Detection of fragrance depended upon ambient temperature and relative humidity. Fragrance life of unharvested inflorescences varied from 3 days up to 4 weeks, whereas that of harvested inflorescences was short, only 1 or 2 days. First generation progeny analyses from 22 crosses between non-fragrant and fragrant parents indicated that multiple genes likely govern the presence of scent in Anthurium.

2003 26 120-124 J. Ehrenberger, Adelheid R. Kuehnle Enhanced histological technique for observation of spathe pigmentation in Anthurium species and hybrids (Buy)
ABSTRACT: A total of 12 Anthurium species and hybrids were examined histologically for pigmentation in spathes using a new method employing vacuum infiltration of spathe tissue with polyethylene glycol (PEG) priorto cross-sectioning. PEG infiltration displaces intercellular air spaces between cells. This method greatly improved the clarity of the cross sections and consequently improved observations of spatial localization of anthocyanins and chloroplasts.